Abstract

Local manifestations of hyperandrogenism are still difficult to diagnose since they are not associated with blood changes of androgens. An increase of androgen receptors (AR) expression level as much as their increase sensitivity to the corresponding ligands may lead to the manifestation of acne, androgenetic alopecia, or hirsutism. It means that in some cases an assessment of the local level of AR expression is necessary for the differential diagnosis and selects the relevant therapy. The traditional laboratory techniques are not available everywhere due to their complexity and laboriousness. In this project, a novel Smart DNA Biosensor, based on RNA-dependent split aptamer for the primary diagnosis of androgen receptors overexpression was developed. This molecular construction is easy to use, has high efficiency and selectivity, dui to its modular structure can be easily optimized for the detection of various targets. Thus, SDB-like sensors may be useful for the rapid differential diagnosis.

Keywords: DNA-based biosensor, androgen receptors, 10-23 deoxyribozymes, RNA cleavage, malachite green aptamer

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Bryushkova, E. A.; Gandalipov, E. R.; Nuzhina, J. V. Beilstein Arch. 2020, 202013. doi:10.3762/bxiv.2020.13.v1

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