Evaluation of hemolytic activity and oxidative stress biomarkers in erythrocytes after exposure to bioactive glass nanoceramics

Ioannis Tsamesidis; Konstantina Kazeli; Georgia Pouroutzidou; Karine Reybier; Antonella Pantaleo; Evgenia Lymperaki; Eleana Kontonasaki

doi:10.3762/bxiv.2019.85.v1

Evaluation of hemolytic activity and oxidative stress biomarkers in erythrocytes after exposure to bioactive glass nanoceramics

Ioannis Tsamesidis ,
Konstantina Kazeli ,
Georgia Pouroutzidou ,
Karine Reybier ,
Antonella Pantaleo ,
Evgenia Lymperaki and
Eleana Kontonasaki

Submitting author affiliation:

Department of Biomedical Sciences, University of Sassari, Sassari, Italy

Beilstein Arch. 2019, 201985. https://doi.org/10.3762/bxiv.2019.85.v1

Published 14 Aug 2019

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SUBJECT AREAS

Show All Preprints Nanotechnology Organic chemistry Bioorganic chemistry / chemical biology Catalysis Chemical methods and reactions Flow and process chemistry Green and sustainable chemistry Materials chemistry Medicinal and pharmaceutical chemistry Nano- and molecular-scale electronics Nano-biomaterials and bioscience Nanomagnetics Nanomaterials, thin films and nanointerfaces Nanomedicine Nanometrology and nanomechanics Nano-optics Nanopatterning, self-assembly and nanofabrication Nanostructures for energy and sensing applications Natural products chemistry Organo main group chemistry Other nanotechnology (unclassified) Other organic chemistry (unclassified) Photochemistry and photovoltaics Physical organic chemistry Supramolecular chemistry

Abstract

The nature of the surface is critical in determining the biological activity of silica powders. A novel correlation between toxicity and surface properties of bioactive glass ceramics (BGCs) synthesized via the sol-gel method was attempted in this study. The behavior of BGCs after their attachment to the surface of red blood cells (RBCs) was evaluated and their toxic effects were determined based on hemolysis, membrane injury via anti-phosphotyrosine immunoblot of Band 3, lipid peroxidation, potential to generate reactive oxygen species, and antioxidant enzyme production. In particular, three BGCs were synthesized at three calcination temperatures (T1=835 °C, T2=1000 °C and T3=1100 °C). Their toxicity based on hemolysis was dose dependent, while BGC-T2 had the best hemocompatibility in compare with the other BGCs. No BGCs in dosages lower than 0.125mg/ml could damage erythrocytes. On the other hand, all BGCs promoted the production of reactive oxygen species in certain concentrations, with the BGC-T2 producing the lowest ROS and increasing the glutathione levels in RBCs protecting their damage. The results suggest that various factors such as size, a probable different proportion of surface silanols, a balanced mechanism between calcium and magnesium cellular uptake or the different crystalline nature may have contributed to this finding; however, future research is needed to clarify the underlying mechanisms.

Keywords: bioactive glass-ceramic nanoparticles, red blood cells, hemolysis, ROS production

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When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Tsamesidis, I.; Kazeli, K.; Pouroutzidou, G.; Reybier, K.; Pantaleo, A.; Lymperaki, E.; Kontonasaki, E. Beilstein Arch. 2019, 201985. doi:10.3762/bxiv.2019.85.v1

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© 2019 Tsamesidis et al.; licensee Beilstein-Institut.
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aromatic	the word “aromatic”
aromatic aldehyde	the word “aromatic” OR “aldehyde”
+aromatic +aldehyde	both words “aromatic” AND “aldehyde”
+aromatic -aldehyde	the word “aromatic” but NOT “aldehyde”
“aromatic aldehyde”	the exact phrase “aromatic aldehyde”
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benzyl~	words that are close to the word “benzyl”, such as “benzoyl” (i.e., fuzzy search)