In vitro effect of hyperthermic Ag and Au Fe3O4 nanoparticles in cancer cells

Hector Katifelis; Iuliia Mukha; Anna Lyberopoulou; Nadiia Vityuk; Matina Grammatikaki; Ievgen Pylypchuk; Foivos Lazaris; Liudmyla Storozhuk; Vassilios Kouloulias; Maria Gazouli

doi:10.3762/bxiv.2019.101.v1

In vitro effect of hyperthermic Ag and Au Fe₃O₄ nanoparticles in cancer cells

Hector Katifelis ,
Iuliia Mukha ,
Anna Lyberopoulou ,
Nadiia Vityuk ,
Matina Grammatikaki ,
Ievgen Pylypchuk ,
Foivos Lazaris ,
Liudmyla Storozhuk ,
Vassilios Kouloulias and
Maria Gazouli

Submitting author affiliation:

Medical School National and Kapodistrian University of Athens, Athens, Greece

Beilstein Arch. 2019, 2019101. https://doi.org/10.3762/bxiv.2019.101.v1

Published 09 Sep 2019

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SUBJECT AREAS

Show All Preprints Nanotechnology Organic chemistry Bioorganic chemistry / chemical biology Catalysis Chemical methods and reactions Flow and process chemistry Green and sustainable chemistry Materials chemistry Medicinal and pharmaceutical chemistry Nano- and molecular-scale electronics Nano-biomaterials and bioscience Nanomagnetics Nanomaterials, thin films and nanointerfaces Nanomedicine Nanometrology and nanomechanics Nano-optics Nanopatterning, self-assembly and nanofabrication Nanostructures for energy and sensing applications Natural products chemistry Organo main group chemistry Other nanotechnology (unclassified) Other organic chemistry (unclassified) Photochemistry and photovoltaics Physical organic chemistry Supramolecular chemistry

Abstract

Metal nanoparticles (NPs) in cancer management have gained great attention the last decade. However, research is needed regarding the targeting potential, the therapeutic effects, in vitro/vivo stability and their cytotoxic effects in order to be successfully incorporated in the clinical practice. In this study, we investigated the anti-cancerous effect of hyperthermic Fe₃O₄ core Αg(Au) _shell NPs constructed with tryptophan as stabilizer and reducing agent. Both NPs have toxic effects after ionization, working as hyperthermic NPs. The toxic effect of hyperthermic NPs is minimized in non-cancerous HEK293 cells. We also studied the expression of hsp-70, bcl-2 and casp-3 in HEK293, HCT116, 4T1 and HUH7 cells and the expression of p53 in HEK293, HCT116 and HUH7. Our results show that Fe₃O_{4 core} Αg(Au) _shell NPs induce apoptosis; 4T1 cells also undergo apoptosis via a p53-independent pathway. However, the role of tryptophan regarding the reduced toxic effects in non-cancerous cells requires further investigation.

Keywords: anti-tumour effect; Fe₃O_{4 core} Αg(Au) _shell nanoparticles; hyperthermia; magnetic nanoparticles; tryptophan

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When a peer-reviewed version of this preprint is available, this information will be updated in the information box above. If no peer-reviewed version is available, please cite this preprint using the following information:

Katifelis, H.; Mukha, I.; Lyberopoulou, A.; Vityuk, N.; Grammatikaki, M.; Pylypchuk, I.; Lazaris, F.; Storozhuk, L.; Kouloulias, V.; Gazouli, M. Beilstein Arch. 2019, 2019101. doi:10.3762/bxiv.2019.101.v1

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© 2019 Katifelis et al.; licensee Beilstein-Institut.
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aromatic	the word “aromatic”
aromatic aldehyde	the word “aromatic” OR “aldehyde”
+aromatic +aldehyde	both words “aromatic” AND “aldehyde”
+aromatic -aldehyde	the word “aromatic” but NOT “aldehyde”
“aromatic aldehyde”	the exact phrase “aromatic aldehyde”
benz*	words which begin with “benz”, such as “benzene” or “benzyl”
benz*yl	words that begin with “benz” and end with “yl”, such as “benzyl” or “benzoyl”
benzyl~	words that are close to the word “benzyl”, such as “benzoyl” (i.e., fuzzy search)